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Marzena E Pazgier Ph.D.

Academic Title: Assistant Professor
Primary Appointment: Biochemistry and Molecular Biology
mpazgier@ihv.umaryland.edu
Location: 725 W. Lombard St. S-431
Phone: (410) 706-4780
Fax: (410) 706-7583

Personal History:

EDUCATION

1996: M.S. Biotechnology, Technical University of Lodz, Poland
1996 to 2001: PhD student, Technical University of Lodz, Poland
2001: Ph.D. Technical Chemistry of Sciences, Technical University of Lodz, Poland,
2002 to 2007: Postdoctoral Research Fellow, NIH, NCI-Frederick, Macromolecular Crystallography Laboratory, Frederick, MD

EMPLOYMENT

2001 to 2002: Research Associate, Technical University of Lodz, Poland
2002 to 2007: Postdoctoral Research Fellow, NIH, NCI-Frederick, Macromolecular Crystallography Laboratory, Frederick, MD
2007 to 2009: Research Associate, Department of Biochemistry and Molecular Biology, Institute of Human Virology, University of Maryland School of Medicine
2009 to present: Assistant Professor, Department of Biochemistry and Molecular Biology, Institute of Human Virology, University of Maryland School of Medicine

Research Interests:

Research in our lab primarily involves the use of x-ray crystallographic techniques to elucidate the structure/function relationships of proteins important for human immune defense, cancer development and viral infections. Proteins currently being studied include: human defensins and cathelicidins, cationic antimicrobial peptides involved in antimicrobial inflammatory responses; the oncoproteins MDM2 and MDMX which negatively regulate the activity and stability of the tumor suppressor protein p53; and the C-terminal domain of HIV-1 capsid protein (CCA), which is involved in HIV-1 assembly and maturation.

Publications:

Pazgier M, Turkiewicz M, Kalinowska H, Bielecki S (2003) "The unique cold-adapted extracellular subtilase from psychrophilic Leucosporidium antarcticum" JMC, part B: Enzymatic, 21:39-42

Turkiewicz M, Pazgier M, Kalinowska H (2003) "Cold-adapted extracellular subtilase from Antarctic, endemic yeast Leucosporidium antarcticum" Extremophiles. 7(6):435-42

Prahl M, Pazgier M, Lockau W, Wlodawer A, Lubkowski J (2004) "Crystal Structure of the Isoaspartyl Peptidase with L-asparaginase activity from E. coli" Acta Crystallogr D. 60:1173-6

Turkiewicz M, Pazgier M, Donachie S.P., Kalinowska H. (2005) "Invertase and alpha-glucosidase production by the endemic Antarctic marine yeast Leucosporidium antarcticum" Polish Polar Research 26(2):125-136

M. Pazgier, D. M. Hoover, D. Yang, W. Lu, J. Lubkowski (2006) "Human beta-defensins" Cell. Mol. Life Sci. 63(11):1294-313

Pazgier M and Lubkowski J (2006) "Expression and purification of recombinant human alpha-defensins in Escherichia coli" Prot. Expr. Purif. 49(1):1-8

Pazgier M, Hoover D., Prahl A., Lubkowski J. (2006) "Studies of biological properties of human beta-defensin 1". J Biol Chem. 2007 Jan 19;282(3):1819-29

Zou G, de Leeuw E, Li C, Pazgier M, Li C, Zeng P, Lu WY, Lubkowski J, Lu W. (2007) Toward understanding the cationicity of defensins. Arg and Lys versus their noncoded analogs. J Biol Chem. 282(27):19653-65

M. Pazgier, X. Li W, Lu, J. Lubkowski (2007) Human defensins: synthesis and structural properties. Current Pharmaceutical Design, 13(30):3096-118.

Rajabi M, de Leeuw E, Pazgier M, Li J, Lubkowski J, Lu W (2008) "The Conserved Salt Bridge in Human {alpha}-Defensin 5 Is Required for Its Precursor Processing and Proteolytic Stability" J Biol Chem, 283(31):21509-18

Li C, Wu Z, Liu M, Pazgier M, Lu W. (2008) Chemically synthesized human survivin does not inhibit caspase-3. Protein Sci. 17(9):1624-9

Pazgier M, Liu M, Zou G, Yuan W, Li C, Li C, Li J, Monbo J, Zella D, Tarasov SG, Lu W. (2009) Structural basis for high-affinity peptide inhibition of p53 interactions with MDM2 and MDMX, Proc Natl Acad Sci U S A.;106(12):4665-70

de Leeuw E, Rajabi M, Zou G, Pazgier M, Lu W. (2009) Selective arginines are important for the antibacterial activity and host cell interaction of human alpha-defensin 5. FEBS Lett.;583(15):2507-12

Wei G, de Leeuw E, Pazgier M, Yuan W, Zou G, Wang J, Ericksen B, Lu WY, Lehrer RI, Lu W. (2009) Through the looking glass: Mechanistic insights from enantiomeric human defensins. J Biol Chem. 2009 Jul 29. [Epub ahead of print]

Li Ch, Pazgier M, Liu M, Lu W-Y, Lu W. (2009) Apamin as a novel template for structure-based rational design of potent peptide activators of p53, Angew. Chem Int Ed Engl [Epub ahead of print]