SOM Faculty Profile : Julie C Hotopp

Julie C Hotopp

Julie C Hotopp Ph.D.

Academic Title: Assistant Professor

Primary Appointment: Microbiology and Immunology

jdhotopp@som.umaryland.edu

Location: HSF-II, S-203B

Phone: (410) 706-5673

Fax: (410) 706-1482

Personal History

Education

May 2002: Ph.D., Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI; Mentor: Dr. Robert Hausinger

May 1997: B.S., magna cum laude, Microbiology and Immunology, Univeristy of Rochester, Rochester, NY

Post Graduate Education and Training

2002-2005: Postdoctoral Fellow, The Institute for Genomic Research, Rockville, MD, Mentor: Dr. Hervé Tettelin.

Professional Experience

2005-2007: Staff Scientist, J. Craig Venter Institute, Rockville, MD.

2007-current: Adjunct Scientist, J. Craig Venter Institute, Rockville, MD

2007-current: Assistant Professor, Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD.

Research Interests

I have worked on numerous whole genome sequencing projects and functional genomics projects for Wolbachia pipientis, Neisseria meningitidis (meningococcus), Streptococcus pneumoniae (pneumococcus), Streptococcus agalactiae (group B Streptococcus), Ehrlichia chaffeensis, Anaplasma phagocytophilum, and Neorickettsia sennetsu. My current research interests are directed toward understanding host-microbe interactions.

One aspect of this work includes examining gene exchange between microbes and their hosts. Although it was thought that DNA could not move from bacteria to higher eukaryotes, we recently found that almost 30% of arthropod and nematode genomes have evidence of DNA transfer from their Wolbachia bacterial endosymbiont. We are characterizing a number of these including the transfer of nearly an entire bacterial genome into the D. ananassae genome.

I am also involved in numerous genomics and microarray studies with Wolbachia pipientis, Neisseria meningitidis, Streptococci, and obligate intracellular bacteria. Some of these projects focus on developing better vaccines while others are targeted at understanding the molecular mechanisms associated with host-bacteria interactions.

Current Funding:

NSF, Genome Sequence of Wolbachia/Drosophila Lateral Gene Transfer,
09/01/08 through 08/31/10.

Our goal is to elucidate the genomic changes resulting from LGT from Wolbachia bacteria to their animal hosts. In the fruit fly Drosophila ananassae, an entire Wolbachia genome has inserted into the host 2L chromosome. We will sequence both the insert and the endosymbiont genome and analyze them using comparative genomic and molecular population genetic techniques to determine the mutational pressures acting on bacterial DNA in a eukaryotic genome.

Publications

Jiang, S. M., N. Ishmael, J. C. Dunning Hotopp, M. Puliti, L. Tissi, N. Kumar, M. J. Cieslewicz, H. Tettelin, and M. R. Wessels (2008) “Variation in the group B Streptococcus CsrRS regulon and effects on pathogenicity.” J Bacteriol 190(6):1956-65.

Bordenstein, S.R., C. Paraskevopoulos, J. C. Dunning Hotopp, P. Sapountzis, N. Lo, C. Bandi, H. Tettelin, J. Werren, and K. Bourtzis. (2009) “Parasitism and mutualism in Wolbachia: what the phylogenomic trees can and cannot say,” Mol Biol Evol 26(1):231-41.

Dunning Hotopp, J. C., M. E. Clark, D. C. S. G. Oliveira, J. M. Foster, P. Fischer, M. C. Munoz Torres, J. D. Giebel, N. Kumar, N. Ishmael, S. Wang, J. Ingram, R. V. Nene, J. Shepard, J. Tomkins, S. Richards, D. J. Spiro, E. Ghedin, B. E. Slatko, H. Tettelin, and J. H. Werren. (2007) "Widespread Lateral Gene Transfer from Intracellular Bacteria to Multicellular Eukaryotes." Science, 317:1753-6.

Ioannidis, P., J. C. Dunning Hotopp, P. Sapountzis, S. Siozios, G. Tasiamis, S. R. Bordenstein, L. Baldo, J. H. Werren, and K. Bourtzis. (2007) "On the Origin of DNA Replication of Wolbachia and Closely Related Bacteria." BMC Genomics 8:182.

Dunning Hotopp, J. C., R. Grifantini, N. Kumar, Y. L. Tzeng, D. Fouts, E. Frigimelica, M. Draghi, M. M. Giuliani, R. Rappuoli, D. S. Stephens, G. Grandi, and H. Tettelin. (2006) "Comparative Genomics of Neisseria meningitidis: Core Genome, Islands of Horizontal Transfer and Pathogen Specific Genes." Microbiology 152:3691-3700.

Baldo, L., J. C. Dunning Hotopp, K. A. Jolley, S. R. Bordenstein, S. A. Biber, R. R. Choudhury, C. Hayashi, M. C. J. Maiden, H. Tettelin, and J. H. Werren. (2006) "A Multilocus Sequence Typing System for the endosymbiont Wolbachia." Appl. Environ. Microbiol. 72(11):7098-7110.

Dunning Hotopp, J. C., M. Lin, R. Madupu, J. Crabtree, S. V. Angiuoli1, J. Eisen, R. Seshadri, Q. Ren, M. Wu, T. R. Utterback, S. Smith, M. Lewis, H. Khouri, C. Zhang, N. Hua, Q. Lin, N. Ohashi, N. Zhi, W. Nelson, L. M. Brinkac, R. J. Dodson, M. J. Rosovitz, J. Sundaram, S. C. Daugherty, T. Davidsen1, A. Durkin, M. Gwinn1, D. H. Haft1, J. D. Selengut, S. A. Sullivan, N. Zafar1, L. Zhou, F. Benahmed, H. Forberger, R. Halpin, S. Mulligan, J. Robinson, Y. Rikihisa, and H. Tettelin. (2006) "Comparative Genomics of Emerging Human Ehrlichiosis Agents." PLoS Genet 2(2):e21.

Dunning Hotopp, J. C., Nelson, W. C., and S. L. Salzberg. (2005) A response to "New names for old strains? Wolbachia wSim is actually wRi." Genome Biology 6:401.

Salzberg, S. L., Dunning Hotopp, J. C., Delcher, A. L., Pop M., Smith, D. R., Eisen, M. B. and W. C. Nelson. (2005) "Correction: Serendipitous discovery of Wolbachia genomes in multiple Drosophila species." Genome Biology 6:R23.

Salzberg, S. L., Dunning Hotopp, J. C., Delcher, A. L., Pop M., Smith, D. R., Eisen, M. B. and W. C. Nelson. (2005) "Serendipitous discovery of Wolbachia genomes in multiple Drosophila species." Genome Biology 6:R23.

Dunning Hotopp, J. C., Auchtung, T. A., Hogan, D. A., and R. P. Hausinger. (2003) "Intrinsic tryptophan fluorescence as a probe of metal and alpha-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase." J. Inorg. Biochem. 93(1-2):66-70.

Dunning Hotopp, J. C., and R. P. Hausinger. (2002) "Probing the 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase substrate-binding site by site-directed mutagenesis and mechanism-based inactivation." Biochemistry 41(31):9787-94.

Elkins, J. M., Ryle, M. J., Clifton I. J., Dunning Hotopp, J. C., Lloyd , J. S., Burzlaff, N. I., Baldwin, J. E., Hausinger, R. P., Roach, P. L. (2002) "X-ray Crystal Structure of Escherichia coli Taurine/alpha-Ketoglutarate Dioxygenase Complexed to Ferrous Iron and Substrates" Biochemistry 41(16):5185-92.

Dunning Hotopp, J. C., and R. P. Hausinger. (2001) "Alternative substrates of 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase." J. Molec. Catalysis B: Enzymatic 15(4-6):155-162.

Dunning, J. C., Y. Ma, and R. E. Marquis. (1998) "Anaerobic killing of oral streptococci by reduced, transitional metal cations." Appl. Environ. Microbiol. 64(1): 27-33.

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