banner.jpg
Bookmark and Share

Publications

Manuscripts

Wahid R, Zafar SJ, McArthur MA, Pasetti MF, Levine MM, Sztein MB. Live oral Salmonella Typhi vaccines Ty21a and CVD 909 induce opsonophagocytic functional antibodies in humans that cross-react with S. Paratyphi A amd S. Paratyphi B. Clinical and Vaccine Immunology, in press. 2014. Temporary PMCID: NIHMS556706.  

Salerno-Goncalves R, Rezwan T, Sztein MB. B cells modulate mucosal associated invariant T cell (MAIT) immune responses. Mucosal Immunity section, Frontiers in Immunology 4:511, 2014. PMCID: PMC3882667. 

Fiorentino M, Levine MM, Sztein MB*, Fasano A*. Effect of wild-type Shigella species and attenuated Shigella vaccine candidates on small intestinal barrier function, antigen trafficking, and cytokine release. PLoS One 9: e85211, 2014. PMCID: PMC3887025. *Joint senior authorship. 

Barry EM, Pasetti MF, Sztein MB, Fasano A, Kotloff KL, Levine MM. Progress and pitfalls in Shigella vaccine research. Nature Reviews Gastroenterology and Hepatology 10: 245-255, 2013. PMCID: PMC3747556. 

Choudhari SP, Pendleton KP, Ramsey JD, Blanchard TG, Picking WD. A systematic approach toward stabilization of CagL, a protein antigen from Helicobacter pylori that is a candidate subunit vaccine. Journal of Pharmaceutical Sciences 102(8): 2508-2519, 2013. PMCID: PMC3903303. 

Davis CL, Wahid R, Toapanta FR, Simon JK, Sztein MB*, Levy D*. Applying mathematical tools to accelerate vaccine development: Modeling Shigella immune dynamics. PLoS One 8(4): e59465, 2013. PMCID: PMC3614931. *Joint senior authorship. 

Eloe-Fadrosh EA, McArthur MA, Seekatz AM, Drabek EF, Rasko DA, Sztein MB*, Fraser CM*. Impact of oral typhoid vaccination on the human gut microbiota and correlations with S. Typhi-specific immunological responses. PLoS One 8(4): e62026, 2013. PMCID: PMC3634757. *Joint senior authorship.  

Eloe-Fadrosh EA, Rasko DA. The human microbiome: From symbiosis to pathogenesis. Annual Review of Medicine 64: 145-163, 2013. PMCID: PMC3731629. 

Fiorentino M, Lammers KM, Levine MM, Sztein MB*, Fasano A*. In vitro intestinal mucosal epithelial responses to wild-type Salmonella Typhi and attenuated typhoid vaccines. Frontiers in Immunology 4: 17, 2013. PMCID: PMC3569575. *Joint senior authorship. 

Fiorentino M, Ding H, Blanchard TG, Czinn SJ, Sztein MB, Fasano A. Helicobacter pylori-induced disruption of monolayer permeability and proinflammatory cytokine secretion in polarized human gastric epithelial cells. Infection and Immunity 81(3): 876-883, 2013. PMCID: PMC3584868. 

McArthur MA, Sztein MB, Edelman R. Prospects for controlling dengue spread: vaccines and vector control. In: Ask the Experts: Dengue fever: transmission, diagnosis & surveillance. Future Science Group, London. e-book. In press, 2013. 

McArthur MA, Sztein MB. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clinical Immunology 146(2): 140-152, 2013. PMCID: PMC3565224. 

Wahid R, Simon JK, Picking WL, Kotloff KL, Levine MM, Sztein MB. Shigella antigen-specific B memory cells are associated with decreased disease severity in subjects challenged with wild-type Shigella flexneri 2a. Clinical Immunology 148(1): 35-43, 2013. PMCID: PMC3679276. 

McArthur MA, Sztein MB, Edelman R. Dengue vaccines: recent developments, ongoing challenges and current candidates. Expert Review of Vaccines 12(8): 933-953, 2013. PMCID: PMC3773977. 

Seekatz AM, Panda A, Rasko DA, Toapanta FR, Eloe-Fadrosh EA, Khan AQ, Liu Z, Shipley ST, DeTolla LJ, Sztein MB, Fraser CM. Differential response of the cynomolgus macque gut microbiota to Shigella infection. PLoS One 8(6): e64212, 2013. PMCID: PMC3673915. 

 Moise L, Gutierrez AH, Bailey-Kellogg C, Terry F, Leng Q, Abdel Hady KM, VerBerkmoes N, Sztein MB, Losikoff P, Martin WD, Rothman A, De Groot AS. The two-faced T cell epitope: Examining the host-microbe interface with Janus Matrix. Human Vaccines & Immunotherapeutics 9(7): 1577-1586, 2013. PMCID: PMC3974887. 

McArthur MA, Sztein MB. Heterogeneity of mutifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization. PLos One 7(6): e38408, 2012. PMCID: PMC3367967. 

Nedrud JG, Czinn SJ, Ding H, Zagorski BM, Redline RW, Twaddell W, Blanchard TG. Lack of genetic influence on the innate inflammatory response to Helicobacter infection of the gastric mucosa. Frontiers in Immunology 3:181, 2012. PMCID: PMC3389333. 

Wahid R, Simon R, Zafar SJ, Levine MM, Sztein MB. Live oral typhoid vaccine Ty21a induces cross-reactive humoral immune responses against Salmonella enterica servovar Paratyhpi A and S. Paratyphi B in humans. Clinical and Vaccine Immunology 19(6): 825-834, 2012. PMCID: PMC3370435. 

Toapanta FR, Bernal PJ, Sztein MB. Diverse phosphorylation patterns of B cell receptor-associated signaling in naive and memory human B cells revealed by phosphoflow, a powerful technique to study signaling at the single cell level. Frontiers in Cellular and Infection Microbiology 2: 128, 2012. PMCID: PMC3473368. 

Lamendella R, VerBerkmoes N, Jansson JK. "Omics" of the mammalian gut - new insights into function. Current Opinions in Biotechnology 23(3): 491-500, 2012. PMC in process. 

Pasetti MF, Simon JK, Sztein MB, Levine MM. Immunology of gut mucosal vaccines. Immunological Reviews 239(1): 125-148, 2011. PMCID: PMC3298192. 

Salerno-Goncalves R, Fasano A, Sztein MB. Engineering of a multicellular organotypic model of intestinal mucosa. Gastroenterology 141(2): e18-e20, 2011. PMCID: PMC3328095. 

Simon JK, Maciel Jr M, Weld ED, Wahid R, Pasetti MF, Picking WL, Kotloff KL, Levine MM, Sztein MB. Antigen-specific IgA B memory cell responses to Shigella antigens elicited in volunteers immunized with live attenuated Shigella flexneri 2a oral vaccine candidates. Clinical Immunology 139(2): 185-192, 2011. PMCID: PMC3078965. 

Ulmer JB, Sztein MB. Promising cutting-edge technologies and tools to accelerate the discovery and development of new vaccines. Editorial overview. Current Opinion in Immunology 23(3): 374-376, 2011. PMC in process. 

Wahid R, Pasetti MF, Milton Jr M, Simon JK, Tacket CO, Levine MM, Sztein MB. Oral priming with Salmonella Typhi vaccine strain CVD 909 followed by parenteral boost with the S. Typhi Vi capsular polysaccharide vaccine induces CD27+ IgD- S. Typhi specific IgA and IgG B memory cells in humans. Clinical Immunology 138(2): 187-200, 2011. PMCID: PMC3035995. 

Salerno-Goncalves R, Wahid R, Sztein MB. Ex vivo kinetics of early and long-term multifunctional HLA-E specific CD8+ cells in volunteers immunized with the Ty21a typhoid vaccine. Clinical Vaccine Immunology 17: 1305-1314, 2010. PMCID: PMC2944457. 

Xu J, Czinn SJ, Blanchard TG. Maintenance of Helicobacter pylori cultures in agar stabs. Helicobacter 15(5): 477-480, 2010. PMCID: PMC3107710.  

Abstracts

De Groot AS, Gutierrez A, He L, Bailey-Kellogg C, Moise L, Terry FE, Losikoff P, Leng Q, Hady KL, Verberkmoes N, Sztein MB, Rothman A, Martin WD. The Two-faced T cell epitope: Relevance to Vaccine Efficacy and Design. Vaccine and Gene Therapy Institute. November 2013. 

Sztein MB, Pollard AJ, Levine MM, Fresnay S, McArthur MA, Wahid R, Toapanta FR, Jones C, Darton TC, Waddington CS, Blohmke CJ, Zhou L, Salerno-Goncalves R.  Complex Cell Mediated Immunity elicited by Salmonella Typhi in Humans.  7th International Conference on Vaccines for Enteric Diseases (VED). November 6-8, 2013, Bangkok, Thailand. 

Wahid R, Zafar SJ, McArthur MA, Pasetti MF, Levine MM, Sztein MB. Live oral typhoid vaccines Ty21a and CVD 909 induce opsonophagocytic functional antibodies in humans which cross react with  S. Paratyphi A and S. Paratyphi B.  7th International Conference on Vaccines for Enteric Diseases (VED). November 6-8, 2013, Bangkok, Thailand.  

Fraser CM, Eloe-Fadrosh E, Seekatz A, Solarno-Aguilar G, Huibbert P, Sztein MB. Probiotics, the Microbiome, and the Host Immune Response:  Insights for Novel Therapeutic Approaches. 7th Annual Vaccine Renaissance Conference, Providence, Rhode Island, October 16-18, 2013. 

Lee L, Corrier KL, Dill BD, Shah M, Hettich RL, Jansson J, Sztein MB, VerBerkmoes NC. Advanced deep metaproteomics methods provide unique insights into the diversity of the human proteome and gut miccrobiota in healthy adults. 7th Annual Vaccine Renaissance Conference, Providence, Rhode Island, October 16-18, 2013. 

Sztein MB, Eloe-Fadrosh E, Seekatz A, McArthur M, Toapanta FR, Panda A,  Rasko DA, Khan AQ, Drabek EF, Liu Z, Shipley ST, DeTolla LJ, Fraser CM. Immune responses to vaccines against enteric pathogens in humans and NHP: Role of the gut microbiome.  7th Annual Vaccine Renaissance Conference, Providence, Rhode Island, October 16-18, 2013. 

Fresnay S, McArthur MA, Wahid R, Salerno-Goncalves R, Toapanta FR, Jones C, Darton TC, Waddington CS, Blohmke CJ, Zhou L, Levine MM, Pollard AJ, Sztein MB. Cell mediated immunity in a human wild-type S. Typhi challenge model.  4th ASM Conference on Salmonella. Boston, MA. October 5-9, 2013. 

Jones C, Darton TC, Waddington CS, Blohmke CJ, Haworth K, Peters A, Kerridge S, Crook D, Lockhart S, Farrar J, Dougan G, Sztein MB, Levine MM, Angus B, Pollard AJ. Blood and stool quantification of bacteria in a controlled human infection model of Salmonella Typhi challenge following vaccination. 4th ASM Conference on Salmonella. Boston, MA. October 5-9, 2013.  

Darton TC, Waddington CS, Jones C, Blohmke C, Sie R, Peters A, Kingsley RA, Dougan G, Lockhart S, Levine MM, Sztein MB, Farrar J, Crook D, Angus B, Pollard AJ. Assessment of the novel oral typhoid vaccine, M01ZH09, using a controlled human infection model of Salmonella Typhi challenge. ISDA 2013. San Francisco, CA. October 2-6, 2013. 

Toapanta FR, Simon J, Barry E, Pasetti M, Levine MM, Kotloff K, Sztein MB. Classic plasmablasts and CD27- plasmablasts infiltrate peripheral blood following CVD 1208S (Shigella) vaccination. 16th International Congress of Mucosal Immunology (ICMI 2013), Vancouver, Canada. July 17-20, 2013. 

Fiorentino M, Ding H, Blanchard TG, Sztein MB, Fasano A. Helicobacter pylori affects gastric epithelial barrier integrity independent of its main virulence factors and induces a polarized host inflammatory cytokine secretion in an in vitro human cell model. Digestive Disease Week 2013, Orlando, FL. May 18 - 21, 2013. 

Fiorentino M, Lammers KM, Sztein MB, Fasano A. Shigella spp. adversely affects paracellular permeability in in vitro and ex vivo models of intestinal mucosa. Digestive Disease Week 2013, Orlando, FL. May 18 - 21, 2013. 

Levine MM, Galen JE, Tennant SM, Pasetti MF, Shirley D, Kotloff KL, Sztein MB.  Live Oral Vaccines to Prevent Typhoid and Paratyphoid Fever. 8th International Conference on Typhoid Fever and Other Invasive Salmonelloses. Dhaka, Bangladesh. March 1-2, 2013. 

Sztein MB, Levine MM, Pollard AJ, Fresnay S, McArthur MA, Jones C, Waddington C, Darton T, Blohmke C, Wahid R, Salerno-Goncalves R. Cell mediated immunity to S. Typhi. 8th International Conference on Typhoid Fever and Other Invasive Salmonelloses, Dhaka, Bangladesh. March 1 - 2, 2013. 

Darton TC, Jones C, Waddington CS, Zhou L, Dougan G, Lockhart S, Levine MM, Sztein MB, Farrar J, Crook D, Angus B, Pollard AJ. Demonstration of primary and asymptomatic DNAaemia in participants challenged with Salmonella Typhi (Quailes strain) during the development of a human model of Typhoid infection. 15th International Congress on Infectious Diseases, Bangkok, Thailand. June 13 - 16, 2012. 

Fiorentino M, Lammers KM, Sztein MB, Fasano A. Salmonella enterica servovar Typhi impairment of epithelial barrier integrity: Effects of wild-type and vaccine strains. 112th American Society for Microbiology Annual Meeting, San Francisco, CA. June 13 - 16, 2012. 

Jones C, Waddington C, Darton T, Bowman J, Farrar J, Dougan G, Levine MM, Lockhart S, Sztein MB, Angus B, Crook D, Pollard AJ. Quantification of antibody secreting cell responses in a human model of Salmonella Typhi infection. 15th International Congress on Infectious Diseases, Bangkok, Thailand. June 13 - 16, 2012. 

Seekatz AM. The effect of an oral live-attenuated Shigella vaccine and wild-type Shigella infection on the intestinal microbiota of cynomolgus monkeys. (Invited speaker.) Maryland Branch American Society for Microbiology Annual Meeting, Baltimore, MD. May 3, 2012.  

Seekatz AM, Eloe-Fadrosh EA, Panda A, Shipley ST, DeTolla LJ, Sztein MB, Fraser CM. The effect of an oral live-attenuated Shigella vaccine and wild-type Shigella infection on the intestinal microbiota of cynomolgus monkeys. Graduate Research Conference, University of Maryland School of Medicine, Baltimore, MD. April 5, 2012.  

Eloe-Fadrosh EA, Seekatz A, Liu Z, Drabek EF, McArthur M, Wahid R, Rasko DA, Sztein MB, Fraser-Liggett CM. Effects of oral immunization with the Ty21a typhoid vaccine on the gut microbiota and local and systemic immune responses. International Human Microbiome Congress, Paris, France. March 19 - 21, 2012. 

Seekatz A, Eloe-Fadrosh E, Panda A, Shipley S, DeTolla L, Sztein MB, Fraser-Liggett C. The effect of an oral live-attenuated Shigella vaccine and wild-type Shigella infection on the intestinal microbiota of cynomolgus monkeys. International Human Microbiome Congress, Paris, France. March 19 - 21, 2012. 

Fiorentino M, Ding H, Blanchard T, Fasano A. Helicobacter pylori-induced impairment of mucosal barrier integrity: A human gastric epithelial cell model. Naspghan, Orlando, FL. October 20 - 23, 2011. 

Toapanta FR, Khan AQ, Panda A, Shipley S, DeTolla L, Chen H, Barry EM, Levine MM, Sztein MB. Immunization with CVD 1256 attenuated Shigella dysenteriae 1 reduces bacterial shedding following wild-type challenge in cynomolgus macaques. International Congress of Mucosal Immunology, Paris, France. July 5 - 9, 2011. 

Salerno-Goncalves R, Fasano A, Sztein MB. Engineering of a multi-cellular organotypic model of the human intestinal mucosa. International Congress of Mucosal Immunology, Paris, France. July 5 - 9, 2011. 

Toapanta FR, Khan AQ, Panda A, Shipley S, DeTolla L, Chen H, Barry EM, Levine MM, Sztein MB. Oral immunization with CVD 1256 attenuated Shigella dysenteriae 1 results in partial protection following wild-type challenge in cynomolgus macaques. Federation of Clinical Immunology Societies, Washington DC. June 23 - 26, 2011.  

Corrier KC, Erickson A, Lamendella R, Cantarel B, Shah M, Fraser-Liggett C, Hettich R, Jansson J, Sztein MB, VerBerkmoes NC. Comparison of direct and indirect extraction methods for deep metaproteomics characterization of the guy microbia. American Society for Mass Spectrometry Annual Conference, Denver, CO. June 5 - 9, 2011. 

Seekatz AM, Liu Z, Panda A, Shipley ST, DeTolla LJ, Sztein MB, Fraser-Liggett CM. 16S rRNA-based pyrosequencing reveals fluctuation in the diversity of bacterial populations over time in the cynomolgus macaque intestinal microbiota. International Human Microbiome Congress, Vancouver, BC, Canada. March 9 - 11, 2011. 

McArthur M, Sztein MB. Presence of multifunctional CD4 and CD8 T cells in response to oral Ty21a typhoid immunization in humans. 10th Annual St. Jude/PIDS Pediatric Infectious Diseases Research Conference. St. Jude Children's Research Hospital, Memphis, TN. February 11 - 12, 2011.  

Seekatz AM, Hsiao WWL, Khan AQ, Panda A, Shipley S, DeTolla L, Levine MM, Sztein MB, Fraser-Liggett CM. Longitudinal analysis of bacterial populations in the cynomolgus monkey gut using 16S rRNA-based pyrosequencing. 110th General Meeting of the American Society for Microbiology, San Diego, CA. May 23 - 27, 2010. 

Seekatz AM, Hsiao WWL, Khan AQ, Panda A, Shipley S, DeTolla L, Levine MM, Barry EM, Sztein MB, Fraser-Liggett CM. The effect of immunization with an oral Shigella vaccine on the gut microbiota in cynomolgus monkeys. 109th General Meeting of the American Society for Microbiology, Philadelphia, PA. May 17 - 21, 2009. 

Presentations

Sztein, MB. Recent advances in the study of CMI to S. Typhi in humans following vaccination or challenge with wild-type organisms. Centre of Clinical Vaccinology and Tropical Medicine (CCVTM).  Oxford, United Kingdom, January 24, 2014. 

Sztein, MB. Complex multifaceted TM, Teff, Treg and MAIT immunity to S. Typhi in humans following vaccination or challenge with wild-type organisms. Weatherall Institute of Molecular Medicine (WIMM).  Oxford, United Kingdom, January 24, 2014. 

Sztein, MB. Cell-mediated immunity to S. Typhi following oral vaccination in a controlled human infection model. Wellcome Trust.  London, United Kingdom, January 23, 2014. 

Sztein, MB. Broad-spectrum Vaccines to Enteric Fevers in Humans: Cross-Protective Immunity. Annual meeting of the Middle Atlantic Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research (MARCE). Turf Valley, Ellicott City, MD.  December 10-11, 2013.  

Sztein, MB. Complex Cell Mediated Immunity elicited by Salmonella Typhi in Humans.  7th International Conference on Vaccines for Enteric Diseases (VED). November 6-8, 2013, Bangkok, Thailand. 

Sztein, MB. Immune responses to vaccines against enteric pathogens in humans and NHP: Role of the gut microbiome.  7th Annual Vaccine Renaissance Conference, Providence, Rhode Island, October 16-18, 2013.  

Sztein, MB. Cell mediated immunity in a human wild-type S. Typhi challenge model.  4th ASM Conference on Salmonella. Boston, MA. October 5-9, 2013. 

Sztein MB. Recent advances on the complex and multifaceted T memory and effector immunity elicited to S. Typhi in humans. 8th International Conference on Typhoid Fever and Other Invasive Salmonelloses, Dhaka, Bangladesh. March 1 - 2, 2013. 

Eloe-Fadrosh EA. The role of the humane microbiome in vaccine efficacy. Phacilitate Washington Vaccine Forum, Washington DC. January 28 - 30, 2013. 

Sztein MB. Update of scientific progress in the Cellular Immunology Section and Flow Cytometry Core Laboratory of the Center for Vaccine Development. Frontiers in Vaccinology, University of Maryland School of Medicine, Baltimore, MD. November 2012. 

Sztein MB. Intricacies in defining immunologically relevant measurements in humans: Systemic immunity as a window into gut mucosal responses. Host Genetics and Immune Response to Enteric Pathogens, Seattle, WA. May 22 - 23, 2012. 

VerBerkmoes, NC. Functional analysis of the human microbiome using community proteomics or proteogenomics. Naval Research Laboratories, Washington DC. February 2012.  

Sztein MB. Role of humoral responses in cross-protection against Salmonella Paratyphi B but not Salmonella Paratyphi A in typhoid vaccinees. Middle Atlantic Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Warrenton, VA. December 19 - 20, 2011. 

Robey, FA. Proteins covalently linked to aluminum oxide nanoparticles as inexpensive, stable mucosally administered vaccine candidates. Vaccines Europe 2011, Brussles, Belgium. November 30 - December 1, 2011. 

VerBerkmoes, NC. Metaproteomics and the human microbiome: Insights into health and disease. Protein DTU Workshop, Copenhagen, Denmark. November 2011. 

VerBerkmoes, NC. Functional analysis of the human microbiome using community proteomics or proteogenomics. National Institutes of Health, Bethesda, MD. August 2011. 

Sztein MB. Recent advances on the complex and multifacted B and T memory and effector immune responses elicited by oral immunization with attenuated S. Typhi in humans. Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom. July 25, 2011. 

VerBerkmoes, NC. Functional analysis of natural microbial consortia using community proteomics or proteogenomics. Oral Microbiome Meeting, Rochester, NY. July 2011. 

Toapanta FR. Oral immunization with CVD 1256 attenuated Shigella dysenteriae 1 results in partial protection following wild-type challenge in cynomolgus macaques. Federation of Clinical Immunology Societies, Washington, DC. June 24, 2011. 

VerBerkmoes, NC. Metaproteomics explored from desert to prairie to human microbiome: Effective protein extraction and peptide identification challenges and potentials. Bacgeco, Greece. June 2011.  

Sztein MB. Host-immune response to bacteria: Enteric pathogens. Neglected Tropical Diseases Workshop, Providence, RI. January 4 - 21, 2011. 

Sztein MB. Oral immunization with attenuated S. Typhi in humans: A window into the multifaceted effector and memory immune responses at mucosal and systemic sites. Memory T Cell Workshop. DAIT, NIAID, NIH, Rockville, MD. September 16 - 17, 2010.