Publications

Manuscripts

Barry EM, Pasetti MF, Sztein MB, Fasano A, Kotloff KL, Levine MM. Progress and pitfalls in Shigella vaccine research. Nature Reviews Gastroenterology and Hepatology 10: 245-255, 2013.

Davis CL, Wahid R, Toapanta FR, Simon JK, Sztein MB*, Levy D*. Applying mathematical tools to accelerate vaccine development: Modeling Shigella immune dynamics. PLoS One 8(4): e59465, 2013. *Joint senior authorship.

Eloe-Fadrosh EA, McArthur MA, Seekatz AM, Drabek EF, Rasko DA, Sztein MB*, Fraser CM*. Impact of oral typhoid vaccination on the human gut microbiota and correlations with S. Typhi-specific immunological responses. PLoS One. In press. 2013. *Joint senior authorship. 

Eloe-Fadrosh EA, Rasko DA. The human microbiome: From symbiosis to pathogenesis. Annual Review of Medicine 64: 145-163, 2013.

Fiorentino M, Lammers KM, Levine MM, Sztein MB*, Fasano A*. In vitro intestinal mucosal epithelial responses to wild-type Salmonella Typhi and attenuated typhoid vaccines. Frontiers in Immunology 4: 17, 2013. PMCID: PMC3569575. *Joint senior authorship.

Fiorentino M, Ding H, Blanchard TG, Czinn SJ, Sztein MB, Fasano A. Helicobacter pylori-induced disruption of monolayer permeability and proinflammatory cytokine secretion in polarized human gastric epithelial cells. Infection and Immunity 81(3): 876-883, 2013. PMCID: PMC3584868.

Lamendella R, VerBerkmoes N, Jansson JK. "Omics" of the mammalian gut - new insights into function. Current Opinions in Biotechnology 23(3): 491-500, 2012.

McArthur MA, Sztein MB. Heterogeneity of mutifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization. PLos One 7(6): e38408, 2012. PMCID: PMC3367967.

McArthur MA, Sztein MB. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clinical Immunology 146(2): 140-152, 2013. PMCID: PMC3565224.

Pasetti MF, Simon JK, Sztein MB, Levine MM. Immunology of gut mucosal vaccines. Immunological Reviews 239(1): 125-148, 2011. PMCID: PMC3298192.

Salerno-Goncalves R, Fasano A, Sztein MB. Engineering of a multicellular organotypic model of intestinal mucosa. Gastroenterology 141(2): e18-e20, 2011. PMCID: PMC3328095.

Salerno-Goncalves R, Wahid R, Sztein MB. Ex vivo kinetics of early and long-term multifunctional HLA-E specific CD8+ cells in volunteers immunized with the Ty21a typhoid vaccine. Clinical Vaccine Immunology 17: 1305-1314, 2010. PMCID: PMC2944457.

Simon JK, Maciel Jr M, Weld ED, Wahid R, Pasetti MF, Picking WL, Kotloff KL, Levine MM, Sztein MB. Antigen-specific IgA B memory cell responses to Shigella antigens elicited in volunteers immunized with live attenuated Shigella flexneri 2a oral vaccine candidates. Clinical Immunology 139(2): 185-192, 2011. PMCID: PMC3078965.

Toapanta FR, Bernal PJ, Sztein MB. Diverse phosphorylation patterns of B cell receptor-associated signaling in naive and memory human B cells revealed by phosphoflow, a powerful technique to study signaling at the single cell level. Frontiers in Cellular and Infection Microbiology 2: 128, 2012. PMCID: PMC3473368.

Ulmer JB, Sztein MB. Promising cutting-edge technologies and tools to accelerate the discovery and development of new vaccines. Editorial overview. Current Opinion in Immunology 23: 1-3, 2011.

Wahid R, Simon JK, Picking WL, Kotloff KL, Levine MM, Sztein MB. Shigella antigen-specific B memory cells are associated with decreased disease severity in subjects challenged with wild-type Shigella flexneri 2a. Clinical Immunology. In press. 2013.

Wahid R, Simon R, Zafar SJ, Levine MM, Sztein MB. Live oral typhoid vaccine Ty21a induces cross-reactive humoral immune responses against Salmonella enterica servovar Paratyhpi A and S. Paratyphi B in humans. Clinical and Vaccine Immunology 19(6): 825-834, 2012. PMCID: PMC3370435.

Wahid R, Pasetti MF, Milton Jr M, Simon JK, Tacket CO, Levine MM, Sztein MB. Oral priming with Salmonella Typhi vaccine strain CVD 909 followed by parenteral boost with the S. Typhi Vi capsular polysaccharide vaccine induces CD27+ IgD- S. Typhi specific IgA and IgG B memory cells in humans. Clinical Immunology 138(2): 187-200, 2011. PMCID: PMC3035995.

Xu J, Czinn SJ, Blanchard TG. Maintenance of Helicobacter pylori cultures in agar stabs. Helicobacter 15(5): 477-480, 2010. PMCID: PMC3107710.
 

Abstracts

Corrier KC, Erickson A, Lamendella R, Cantarel B, Shah M, Fraser-Liggett C, Hettich R, Jansson J, Sztein MB, VerBerkmoes NC. Comparison of direct and indirect extraction methods for deep metaproteomics characterization of the guy microbia. American Society for Mass Spectrometry Annual Conference, Denver, CO. June 5 - 9, 2011.

Darton TC, Jones C, Waddington CS, Zhou L, Dougan G, Lockhart S, Levine MM, Sztein MB, Farrar J, Crook D, Angus B, Pollard AJ. Demonstration of primary and asymptomatic DNAaemia in participants challenged with Salmonella Typhi (Quailes strain) during the development of a human model of Typhoid infection. 15th International Congress on Infectious Diseases, Bangkok, Thailand. June 13 - 16, 2012.

Eloe-Fadrosh EA, Seekatz A, Liu Z, Drabek EF, McArthur M, Wahid R, Rasko DA, Sztein MB, Fraser-Liggett CM. Effects of oral immunization with the Ty21a typhoid vaccine on the gut microbiota and local and systemic immune responses. International Human Microbiome Congress, Paris, France. March 19 - 21, 2012.

Fiorentino M, Ding H, Blanchard TG, Sztein MB, Fasano A. Helicobacter pylori affects gastric epithelial barrier integrity independent of its main virulence factors and induces a polarized host inflammatory cytokine secretion in an in vitro human cell model. Digestive Disease Week 2013, Orlando, FL. May 18 - 21, 2013.

Fiorentino M, Lammers KM, Sztein MB, Fasano A. Shigella spp. adversely affects paracellular permeability in in vitro and ex vivo models of intestinal mucosa. Digestive Disease Week 2013, Orlando, FL. May 18 - 21, 2013.

Fiorentino M, Lammers KM, Sztein MB, Fasano A. Salmonella enterica servovar Typhi impairment of epithelial barrier integrity: Effects of wild-type and vaccine strains. 112th American Society for Microbiology Annual Meeting, San Francisco, CA. June 13 - 16, 2012.

Fiorentino M, Ding H, Blanchard T, Fasano A. Helicobacter pylori-induced impairment of mucosal barrier integrity: A human gastric epithelial cell model. Naspghan, Orlando, FL. October 20 - 23, 2011.

Jones C, Waddington C, Darton T, Bowman J, Farrar J, Dougan G, Levine MM, Lockhart S, Sztein MB, Angus B, Crook D, Pollard AJ. Quantification of antibody secreting cell responses in a human model of Salmonella Typhi infection. 15th International Congress on Infectious Diseases, Bangkok, Thailand. June 13 - 16, 2012.

McArthur M, Sztein MB. Presence of multifunctional CD4 and CD8 T cells in response to oral Ty21a typhoid immunization in humans. 10th Annual St. Jude/PIDS Pediatric Infectious Diseases Research Conference. St. Jude Children's Research Hospital, Memphis, TN. February 11 - 12, 2011. 

Salerno-Goncalves R, Fasano A, Sztein MB. Engineering of a multi-cellular organotypic model of the human intestinal mucosa. International Congress of Mucosal Immunology, Paris, France. July 5 - 9, 2011.

Seekatz AM. The effect of an oral live-attenuated Shigella vaccine and wild-type Shigella infection on the intestinal microbiota of cynomolgus monkeys. (Invited speaker.) Maryland Branch American Society for Microbiology Annual Meeting, Baltimore, MD. May 3, 2012. 

Seekatz AM, Eloe-Fadrosh EA, Panda A, Shipley ST, DeTolla LJ, Sztein MB, Fraser CM. The effect of an oral live-attenuated Shigella vaccine and wild-type Shigella infection on the intestinal microbiota of cynomolgus monkeys. Graduate Research Conference, University of Maryland School of Medicine, Baltimore, MD. April 5, 2012. 

Seekatz A, Eloe-Fadrosh E, Panda A, Shipley S, DeTolla L, Sztein MB, Fraser-Liggett C. The effect of an oral live-attenuated Shigella vaccine and wild-type Shigella infection on the intestinal microbiota of cynomolgus monkeys. International Human Microbiome Congress, Paris, France. March 19 - 21, 2012.

Seekatz AM, Liu Z, Panda A, Shipley ST, DeTolla LJ, Sztein MB, Fraser-Liggett CM. 16S rRNA-based pyrosequencing reveals fluctuation in the diversity of bacterial populations over time in the cynomolgus macaque intestinal microbiota. International Human Microbiome Congress, Vancouver, BC, Canada. March 9 - 11, 2011.

Seekatz AM, Hsiao WWL, Khan AQ, Panda A, Shipley S, DeTolla L, Levine MM, Sztein MB, Fraser-Liggett CM. Longitudinal analysis of bacterial populations in the cynomolgus monkey gut using 16S rRNA-based pyrosequencing. 110th General Meeting of the American Society for Microbiology, San Diego, CA. May 23 - 27, 2010.

Seekatz AM, Hsiao WWL, Khan AQ, Panda A, Shipley S, DeTolla L, Levine MM, Barry EM, Sztein MB, Fraser-Liggett CM. The effect of immunization with an oral Shigella vaccine on the gut microbiota in cynomolgus monkeys. 109th General Meeting of the American Society for Microbiology, Philadelphia, PA. May 17 - 21, 2009.

Sztein MB, Levine MM, Pollard AJ, Fresnay S, McArthur MA, Jones C, Waddington C, Darton T, Blohmke C, Wahid R, Salerno-Goncalves R. Cell mediated immunity to S. Typhi. 8th International Conference on Typhoid Fever and Other Invasive Salmonelloses, Dhaka, Bangladesh. March 1 - 2, 2013.

Toapanta FR, Khan AQ, Panda A, Shipley S, DeTolla L, Chen H, Barry EM, Levine MM, Sztein MB. Immunization with CVD 1256 attenuated Shigella dysenteriae 1 reduces bacterial shedding following wild-type challenge in cynomolgus macaques. International Congress of Mucosal Immunology, Paris, France. July 5 - 9, 2011.

Toapanta FR, Khan AQ, Panda A, Shipley S, DeTolla L, Chen H, Barry EM, Levine MM, Sztein MB. Oral immunization with CVD 1256 attenuated Shigella dysenteriae 1 results in partial protection following wild-type challenge in cynomolgus macaques. Federation of Clinical Immunology Societies, Washington DC. June 23 - 26, 2011.

 

Presentations

Eloe-Fadrosh EA. The role of the humane microbiome in vaccine efficacy. Phacilitate Washington Vaccine Forum, Washington DC. January 28 - 30, 2013.

Robey, FA. Proteins covalently linked to aluminum oxide nanoparticles as inexpensive, stable mucosally administered vaccine candidates. Vaccines Europe 2011, Brussles, Belgium. November 30 - December 1, 2011.

Sztein MB. Recent advances on the complex and multifaceted T memory and effector immunity elicited to S. Typhi in humans. 8th International Conference on Typhoid Fever and Other Invasive Salmonelloses, Dhaka, Bangladesh. March 1 - 2, 2013.

Sztein MB. Update of scientific progress in the Cellular Immunology Section and Flow Cytometry Core Laboratory of the Center for Vaccine Development. Frontiers in Vaccinology, University of Maryland School of Medicine, Baltimore, MD. November 2012.

Sztein MB. Intricacies in defining immunologically relevant measurements in humans: Systemic immunity as a window into gut mucosal responses. Host Genetics and Immune Response to Enteric Pathogens, Seattle, WA. May 22 - 23, 2012.

Sztein MB. Role of humoral responses in cross-protection against Salmonella Paratyphi B but not Salmonella Paratyphi A in typhoid vaccinees. Middle Atlantic Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Warrenton, VA. December 19 - 20, 2011.

Sztein MB. Recent advances on the complex and multifacted B and T memory and effector immune responses elicited by oral immunization with attenuated S. Typhi in humans. Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom. July 25, 2011.

Sztein MB. Host-immune response to bacteria: Enteric pathogens. Neglected Tropical Diseases Workshop, Providence, RI. January 4 - 21, 2011.

Sztein MB. Oral immunization with attenuated S. Typhi in humans: A window into the multifaceted effector and memory immune responses at mucosal and systemic sites. Memory T Cell Workshop. DAIT, NIAID, NIH, Rockville, MD. September 16 - 17, 2010.

Toapanta FR. Oral immunization with CVD 1256 attenuated Shigella dysenteriae 1 results in partial protection following wild-type challenge in cynomolgus macaques. Federation of Clinical Immunology Societies, Washington, DC. June 24, 2011.

VerBerkmoes, NC. Functional analysis of the human microbiome using community proteomics or proteogenomics. Naval Research Laboratories, Washington DC. February 2012.

VerBerkmoes, NC. Metaproteomics and the human microbiome: Insights into health and disease. Protein DTU Workshop, Copenhagen, Denmark. November 2011.

VerBerkmoes, NC. Functional analysis of the human microbiome using community proteomics or proteogenomics. National Institutes of Health, Bethesda, MD. August 2011.

VerBerkmoes, NC. Functional analysis of natural microbial consortia using community proteomics or proteogenomics. Oral Microbiome Meeting, Rochester, NY. July 2011.

VerBerkmoes, NC. Metaproteomics explored from desert to prairie to human microbiome: Effective protein extraction and peptide identification challenges and potentials. Bacgeco, Greece. June 2011.